Isolation, Identification Total Alkaloid Tempuyung (Sonchus Arvensis Linn) Leaf and Cytotoxic Activity with BSLT (Brine Shrimp Lethality Test) Method

Tempuyung (Sonchus arvensis L.) from asteraceae tribe of the medicine plants that have some compounds secondary metabolic, one of them is Alkaloid. There has been done isolation of alkaloid compound to ethanol extract of tempuyung dry leave using extraction and preparative TLC using eluen n-hexane: ethyl acetate: ethanol (30:2:1). Analysis carried out on pure alkaloid isolate using method of UV-Vis spectrophotometer, FTIR and LC-MS. Based on the analysis using UV-Vis spectrophotometer, FTIR and LC-MS can be a compound known alkaloid contained in the leaves tempuyung including alkaloids with the basic framework isokuinolin having absorption at a wavelength of 225 nm, 253 nm, and 352 nm, has a functional group C=N, O-H, C-O, conjugated C=C, C=O, CH₂, CH₃ and molecular weight compounds at 444.84 g/mol. The result of cytotoxic test using method of BSLT generates rate of LC₅₀ from ethanol extract and total alkaloid isolate respectively 61.410 ppm and 523.634 ppm , it means the ethanol extract is less toxic and total alkaloid isolate is not toxic.

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